zirconium beads dna extraction

Tissue grinder with zirconium beads

● 0.1mm diameter zirconia beads for bacteria;

● Select 0.5mm diameter zirconia beads for yeast or fungi;

● Zirconia beads of 1.0mm diameter are selected for most tissues;

● 2.0mm zirconia beads for skin or "soft" plant materials;

● Use the same diameter for fairly hard or fibrous tissues, but you need to choose a higher density bead. For example, the researchers chose 0.1mm zirconia beads to break spores or 2.3mm zirconia beads to extract hard fibrous plant material such as monocotyledon leaves.

Guide:

1. Bacteria recommend using zirconia beads with a diameter of 0.1mm

2. Yeast/fungi please choose zirconia beads with a diameter of 0.5mm

3. Beads with a diameter of 1.0mm are selected for tissue blocks

4. Please use 2.5mm diameter beads for skin tissue and plant tissue

5. In actual work, researchers often choose beads that are close to the particle size of the sample, and the density is larger. For example, broken spores often use 0.1mm zirconia-silica beads; Broken plant fiber tissue is often used 2.5mm zirconia beads or stainless steel beads

6. Preliminary studies we have have shown that the crushing of plant and animal tissue using angular particles is more time efficient than using smooth beads

Most of the time, there is no need to clean the glass beads or ceramic beads you just bought, because they are only contaminated with carbon black when they are new, carbon black is inert, and its presence does not affect the results of your experiment. In addition, the carbon black is quickly removed by centrifugation or filtration after crushing. Remember, do not use acid to clean beads. You can soak the used beads in a laboratory cleaner overnight, rinse them off with running water, and then rinse them with distilled water. Place the washed beads in a stainless steel tray or glass tray in an oven at 40-70 degrees Celsius to dry.